Validating Real-Time Quantitative RT-PCR Amplicons From Linearly Amplified Primitive Hematopoietic Progenitor mRNA
نویسندگان
چکیده
منابع مشابه
Validating Real-Time Quantitative RT-PCR Amplicons From Linearly Amplified Primitive Hematopoietic Progenitor mRNA
Objective. Low gene expression in rare cell subpopulations can make it difficult to identify transcripts using real time quantitative RT-PCR (qRT-PCR). Transcript number can be increased using linear amplification, but this technique amplifies the 3’ end of mRNA, imposing severe limitations on qRT-PCR primer design. Artifacts such as primer-dimers, introduced by these limitations, may interfere...
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ژورنال
عنوان ژورنال: The Open Circulation & Vascular Journal
سال: 2009
ISSN: 1877-3826
DOI: 10.2174/1877382600801010001